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Serodetection of btb using multiantigens



Diagnostic Microbiology and Infectious Disease - Vol 46, Iss3 , July 2003, pp197-203


Rena Greenwald, Javan Esfandiari, Sandrine Lesellier, Raymond Houghton, John Pollock, Claus Aagaard, Peter Andersen, R. Glyn Hewinson, Mark Chambers, and Konstantin Lyashchenko from the Chembio Diagnostic Systems, Inc., USA; Veterinary Laboratories Agency, Surrey; Corixa Corporation, Washington, USA; Department of Agriculture and Rural Development, Belfast; and the Department of TB Immunology, Copenhagen, Denmark.


Despite attempts to control bovine tuberculosis, the incidence of disease in Great Britain continues to rise. In GB, the European badger (Meles meles) is a reservoir of infection with Mycobacterium bovis. In an effort to improve the serodetection of badger tuberculosis, we examined sera from M. bovis culture-positive and culture-negative badgers for their ability to recognize M. bovis antigens, using a multi-antigen print immunoassay (MAPIA). Depending on the antigens used in the MAPIA, the assay had a sensitivity of 49–59% and a specificity of 84-88% Results from the MAPIA were used to select antigens for the development of a lateral-flow immunoassay. This so-called ‘Rapid Test’ used 5μl of serum and gave unambiguous results within 10 min. When applied to 178 badger sera, the Rapid Test had a sensitivity of 53% and a specificity of 95%. This represented an improvement over the performance of the existing ELISA Test, which had a sensitivity of 47% and a specificity of 89% on the same sera. This is the first report of a diagnostic test for badger tuberculosis that can be performed alongside the captive animal.

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